B3GNT3

Gene Symbol B3GNT3
Entrez Gene 10331
Alt Symbol B3GAL-T8, B3GN-T3, B3GNT-3, HP10328, TMEM3, beta3Gn-T3
Species Human
Gene Type protein-coding
Description UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
Other Description N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3|UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8|UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 8|beta-1,3-GalTase 8|beta-1,3-Gn-T3|beta-1,3-N-acetylglucosaminyltransferase bGnT-3|beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase|beta-3-Gx-T8|beta3GalT8|core 1 extending beta-1,3-N-acetylglucosaminyltransferase|core1-beta3GlcNAcT|putative type II membrane protein|transmembrane protein 3
Swissprots Q9C0J2 Q8WWR6 Q6NWU9 Q9Y2A9 B2RAS4 Q6NXU9
Accessions EAW84631 Q9Y2A9 AB015630 BAA76497 AB049585 BAB21531 AF293973 AAK00849 AJ130847 CAC45044 AJ278961 CAC82374 AK075434 AK075466 BAG52147 AK225421 AK225478 AK314323 BAG36971 AW848963 AY358955 AAQ89314 BC066876 AAH66876 BC067423 AAH67423 DA385410 DB104034 HQ447547 ADQ32033 XM_011527626 XP_011525928 XM_011527627 XP_011525929 NM_014256 NP_055071
Function Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides (PubMed:11042166). Also acts as a core1-1,3-N- acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6- sulfo sialyl Lewis x on extended core1 O-glycans (PubMed:11439191). {ECO:0000269|PubMed:11042166, ECO:0000269|PubMed:11439191}.
Subcellular Location Golgi apparatus membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
Tissue Specificity Expressed in colon, jejunum, stomach, esophagus, placenta and trachea. {ECO:0000269|PubMed:11042166}.
Top Pathways Glycosphingolipid biosynthesis - lacto and neolacto series

β3Gn-T3 (D-12) - sc-131362 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-B3GNT3 antibody - ab190458 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunoprecipitation of 20 μg HeLa lysate labelling B3GNT3 with ab190458 at 6 μg/mg lysate. Secondary antibody used at 1 μg/ml dilution.

Anti-B3GNT3 antibody - ab96267 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-B3GNT3 antibody (ab96267) at 1/1000 dilution + 293T whole cell lysate at 30 µg

Anti-B3GNT3 antibody - ab70156 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-B3GNT3 antibody (ab70156) at 1/500 dilutionLane 1 : B3GNT3 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-B3GNT3 / TMEM3 Antibody - LS-C306473 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-B3GNT3 / TMEM3 Antibody (aa135-208) - LS-C306474 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-B3GNT3 / TMEM3 Antibody - LS-C309567 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-B3GNT3 / TMEM3 Antibody (aa50-100) - LS-C291076 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human B3GNT3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-B3GNT3 antibody used for IP at 6 ug per reaction. B3GNT3 was also immunoprecipitated by rabbit anti-B3GNT3 antibody BL16626. For blotting immunoprecipitated B3GNT3, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-B3GNT3 / TMEM3 Antibody (aa122-338) - LS-C186034 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
B3GNT3 antibody detects B3GNT3 protein at cytosol on colon by immunohistochemical analysis. Sample: Paraffin-embedded colon. B3GNT3 antibody dilution:1:500.

B3GNT3 Antibody - NBP1-32539 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: B3GNT3 Antibody [NBP1-32539] - Sample (50 ug of whole cell lysate) A: mouse stomach 10% SDS PAGE; antibody diluted at 1:1000.

B3GNT3 Antibody - NBP1-88953 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: B3GNT3 Antibody [NBP1-88953] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

B3GNT3 Antibody - H00010331-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

B3GNT3 Antibody - H00010331-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: B3GNT3 Antibody [H00010331-A01] - B3GNT3 polyclonal antibody (A01), Lot # 060519JCS1 Western Blot analysis of B3GNT3 expression in HeLa ( Cat # L013V1 ).

B3GNT3 Antibody - H00010331-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: B3GNT3 Antibody [H00010331-D01P] - Analysis of B3GNT3 expression in mouse liver.

B3GNT3 Antibody (1A2) - H00010331-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A2
Immunocytochemistry/Immunofluorescence: B3GNT3 Antibody (1A2) [H00010331-M01] - Analysis of monoclonal antibody to B3GNT3 on HeLa cell. Antibody concentration 10 ug/ml.

Anti-B3GNT3 antibody produced in mouse - SAB1406975 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-B3GNT3: <B>Cat. No. SAB1406975</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~40.9 kDa using B3GNT3 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-B3GNT3 antibody produced in rabbit - HPA024298 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-B3GNT3: <B>Cat. No. HPA024298</B>: Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Monoclonal Anti-B3GNT3 antibody produced in mouse - SAB1403060 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A2
<B>ELISA</B><BR/>Monoclonal Anti-B3GNT3: <B>Cat. No. SAB1403060</B>: Detection limit for recombinant GST tagged B3GNT3 is 0.3 ng/mL.

beta3Gn T3 antibody - orb69866 from Biorbyt

Prices $723.00
Sizes 100 μl
Host Rabbit
Clonality Polyclonal
Western Blot analysis of A:293T whole cell lysate using beta3Gn T3 antibody

B3GNT3 antibody - 70R-15947 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

B3GNT3 antibody - 70R-13239 from Fitzgerald

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

B3GNT3 Polyclonal Antibody - A4407 from ABclonal

Prices $200.00, $480.00
Sizes 50 µg, 200 µg
Host Rabbit
Clonality Polyclonal

B3GNT3 antibody (OAGA00730) - OAGA00730 from Aviva Systems Biology

Prices $340.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

B3GNT3 Antibody - abx003289 from Abbexa

Host Rabbit
Clonality Polyclonal
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