EIF2AK3

Gene Symbol EIF2AK3
Entrez Gene 9451
Alt Symbol PEK, PERK, WRS
Species Human
Gene Type protein-coding
Description eukaryotic translation initiation factor 2-alpha kinase 3
Other Description PRKR-like endoplasmic reticulum kinase|eukaryotic translation initiation factor 2 alpha kinase 3|hsPEK|pancreatic EIF2-alpha kinase
Swissprots A0AVH2 Q53SB1 Q53QY0 Q9NZJ5 A0AVH1 B2RCU9 O95846
Accessions AAF91480 AAY14777 AAY24331 EAW77068 EAW77069 Q9NZJ5 AF110146 AAD19961 AF193339 AAF61199 AI650734 AK128659 BAG54707 AK308312 AK315287 BAG37696 BC040505 BC126354 AAI26355 BC126356 AAI26357 BQ014181 CN285475 CR749382 CAH18234 DA990609 DB287944 XM_005264649 XP_005264706 XR_939749 NM_004836 NP_004827
Function Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function (By similarity). {ECO:0000250}.
Subcellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Tissue Specificity Ubiquitous. A high level expression is seen in secretory tissues.
Top Pathways Alzheimer's disease, Protein processing in endoplasmic reticulum, Hepatitis C, Measles, Influenza A

PERK (C33E10) Rabbit mAb - 3192 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C33E10
Western blot analysis of extracts from various cell types using PERK (C33E10) Rabbit mAb.

PERK (D11A8) Rabbit mAb - 5683 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D11A8
Western blot analysis of extracts from various cell lines using PERK (D11A8) Rabbit mAb.

Phospho-PERK (Thr980) Antibody - 3191 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

PERK (B-5) - sc-377400 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PERK (B-5): sc-377400. Western blot analysis of PERK expression in nocodazole treated HeLa whole cell lysate.

PERK (N-18) - sc-9479 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PERK (N-18): sc-9479. Western blot analysis of PERK expression in K-562 (A) and Daudi (B) whole cell lysates.

PERK (C-16) - sc-9481 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PERK (C-16): sc-9481. Western blot analysis of PERK expression in Hep G2 whole celllysate.

PERK (H-300) - sc-13073 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PERK (H-300): sc-13073. Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic and nuclear staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.

p-PERK (Thr 981) - sc-32577 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-PERK (Thr 981): sc-32577. Western blot analysis of PERK phosphorylation in HeLa (A) and Thapsigargin treated HeLa (B) whole cell lysates.

Anti-PERK (phospho T980) antibody - ab156919 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PERK (phospho T980) antibody (ab156919) at 1 µg/mlLane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate with Rat PERK (phospho T980) peptide (ab195519) at 1 µg/mlLane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate with Rat PERK peptide (ab195521) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PERK (phospho T981) antibody - ab192591 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labeling PERK (phospho T981) with ab192591 at a 1/50 dilution. The image on the right shows the same antibody preincubated with blocking peptide.

Anti-PERK antibody - ab115617 from Abcam

Prices $387.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
IHC-P image of PERK staining on human cortical sections using ab115617 (1:1500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 2 hour at 20°C. ab115617 was diluted 1:500 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500)See Abreview

Anti-PERK antibody - ab65142 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of HepG2 cells using ab65142 at a 1/500-1/1000 dilution. Left image untreated. Right image treated with immunising peptide.

Anti-PERK antibody - ab79483 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC-P image of PERK staining on human cortical sections using ab79483 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab79483 was diluted 1:500 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500)See Abreview

Anti-PERK antibody - ab77654 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
IHC-P image of PERK staining on human cortical sections using ab77654 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 2 hour at 20°C. ab77654 was diluted 1:500 and incubated with the sections for 18 hours at 4°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500) See Abreview

Anti-PERK antibody [5G5] - ab105929 from Abcam

Host Mouse
Clonality Monoclonal
Clone 5G5
All lanes : Anti-PERK antibody [5G5] (ab105929) at 1/500 dilutionLane 1 : Un-transfected HEK293Lane 2 : PERK-hIgGFc transfected HEK293

Anti-EIF2AK3 / PERK Antibody (phospho-Thr980) - LS-C38147 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal

Anti-EIF2AK3 / PERK Antibody (aa29-230) - LS-C38148 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-EIF2AK3 / PERK Antibody (AP) - LS-C222352 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EIF2AK3 / PERK Antibody (APC) - LS-C222353 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EIF2AK3 / PERK Antibody (aa148-175) - LS-C101136 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-EIF2AK3 / PERK Antibody (FITC) - LS-C222354 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EIF2AK3 / PERK Antibody (phospho-Thr981) - LS-C199431 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human prostate carcinoma, using PEK/PERK (Phospho-Thr981) Antibody. The sample on the right was incubated with synthetic peptide.

Anti-EIF2AK3 / PERK Antibody (Biotin) - LS-C222355 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EIF2AK3 / PERK Antibody (PE) - LS-C222357 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EIF2AK3 / PERK Antibody (Azide-free, HRP) - LS-C222356 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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