DDX59

Gene Symbol DDX59
Entrez Gene 83479
Alt Symbol OFD5, ZNHIT5
Species Human
Gene Type protein-coding
Description DEAD (Asp-Glu-Ala-Asp) box polypeptide 59
Other Description DEAD box protein 59|probable ATP-dependent RNA helicase DDX59|zinc finger HIT domain-containing protein 5
Swissprots Q9H0W3 Q6PJL2 Q8IVW3 Q5T1V6
Accessions EAW91318 EAW91319 Q5T1V6 AK299219 BAH12972 AK307569 AL136611 CAB66546 AM393451 CAL38329 AM393546 CAL38423 BC014183 AAH14183 BC020201 BC041801 AAH41801 BU754283 DB483119 XM_005245519 XP_005245576 XM_005245520 XP_005245577 XM_005245521 XP_005245578 XM_006711562 XP_006711625 XM_011510035 XP_011508337 XR_921966 XR_921967 NM_031306 NM_001031725 NP_001026895
Subcellular Location Cytoplasm {ECO:0000269|PubMed:23972372}. Nucleus {ECO:0000269|PubMed:23972372}. Note=Exhibits granular localization in the nucleus, as well as in the cytoplasm.
Tissue Specificity Expressed in fibroblasts (at protein level). {ECO:0000269|PubMed:23972372}.

DDX59 (N-16) - sc-104192 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DDX59 (N-16): sc-104192. Western blot analysis of DDX59 expression in HeLa nuclear extract.

DDX59 (C-3) - sc-514439 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DDX59 (C-3): sc-514439. Western blot analysis of DDX59 expression in HeLa nuclear extract (A) and HeLa (B), MCF7 (C), HCT-116 (D) and A549 (E) whole cell lysates.

DDX59 (D-13) - sc-104191 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-DDX59 antibody - ab109592 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDX59 antibody (ab109592) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-DDX59 antibody - ab69521 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-DDX59 antibody (ab69521) at 1/500 dilution + human liver tissue lysate at 25 µgSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Anti-DDX59 antibody - ab85663 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-DDX59 antibody (ab85663) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-DDX59 antibody [2G1] - ab156771 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G1
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DDX59 with ab156771 at 1/500 dilution.

Anti-DDX59 antibody [3F8] - ab131660 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F8
All lanes : Anti-DDX59 antibody [3F8] (ab131660) at 1 µg/mlLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 :  HEK293T cell lysate transfected with pCMV6-ENTRY DDX59 cDNALysates/proteins at 5 µg per lane.

Anti-DDX59 Antibody (aa569-619) - LS-C289733 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human DDX59 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DDX59 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. DDX59 was also immunoprecipitated by rabbit anti-DDX59 antibody BL10944, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Anti-DDX59 Antibody (clone 2G1) - LS-C175381 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G1
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DDX59 mouse monoclonal antibody.

Anti-DDX59 Antibody (clone 3E9) - LS-C175280 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E9
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX59 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX59.

Anti-DDX59 Antibody (clone 1F11) - LS-C175283 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F11
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX59 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX59.

Anti-DDX59 Antibody (aa151-200) - LS-C101634 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DDX59 antibody LS-C101634 Western blot of MCF7 cell lysate.

Anti-DDX59 Antibody (clone 1F8) - LS-C175293 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F8
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX59 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX59.

Anti-DDX59 Antibody (clone 3C3) - LS-C175287 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3C3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX59 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX59.

Anti-DDX59 Antibody (clone 3F8) - LS-C175297 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F8
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX59 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX59.

Anti-DDX59 Antibody - LS-C307205 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-DDX59 Antibody (clone 2C1) - LS-C175294 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX59 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX59.

DDX59 Antibody - NBP2-13909 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: DDX59 Antibody [NBP2-13909] - Staining of human cell line U-2 OS shows positivity in cytoskeleton (actin filaments).

DDX59 Antibody - NBP2-16143 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DDX59 Antibody - NBP2-16142 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DDX59 Antibody - H00083479-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: DDX59 Antibody [H00083479-B01P] - Analysis of DDX59 expression in human liver.

Anti-DDX59 antibody produced in mouse - SAB1400723 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Polyclonal Anti-DDX59, antibody produced in mouse, purified immunoglobulin: <B>Cat. No. SAB1400723</B>: Antibody concentration: 1.0 ~ 5.0 μg/mL. Specific band of ~68.8 kDa using human liver lysate.

Anti-DDX59 (76-90) antibody produced in rabbit - SAB1103211 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human DDX59 was separated on SDS-PAGE, blotted with Anti-DDX59 (76-90) (<B>Cat. No. SAB1103211</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000 <BR/>2. Antibody dilution 1:1,000 + DDX59 immunizing peptide (human, 76-90)

Monoclonal Anti-DDX59 antibody produced in mouse - SAB4100472 from Sigma-Aldrich

Prices $377.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone DDX59 -B413
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