Immunofluorescent analysis of MCF7 cells, serum-starved (left) or insulin-treated (right), using PathScan® Signaling Nodes Multiplex IF Kit. Red = Phospho-Akt (Ser473), green = Phospho-p44/42 (Thr202/Tyr204), and blue pseudocolor = Phospho-S6 (Ser235/236).