Western blot analysis of Cdc6 phosphorylation in untreated (A,D), serum starved and treated (B,E) and serum starved, serum treated and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-Cdc6 (Ser 54)-R: sc-12920-R (A,B,C) and Cdc6 (D-1): sc-13136 (D,E,F).