ab118171 ligand binding and displacement. Top (A, C, E): ab118171 ligand (100 nM) binding to three different live cell lines expressing β1 (A), β2 (C) and β3 (E) adrenoceptors. Bottom (B, D, F): Binding blocked in the same three cell lines using the unlabelled competitor ICI118551 at 1 µM (β1: B, β2: D) or SR59230A at 1 µM (β3: F). Nuclei have been counter-stained with Hoechst.