Active human Furin protein fragment
| Name | Active human Furin protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab167741 |
| Category | Protein |
| Prices | $410.00 |
| Sizes | 20 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Tag/Conjugation | His tag C-Terminus |
| Purity | >95% by SDS-PAGE . ab167741 was lyophilized from 0.22 µm filtered solution. |
| Bioactivity | Measured by its ability to cleave the fluorogenic peptide substrate p – Glu – Arg – Thr – Lys – Arg – AMC. The bioactivity was measured in 100µL reaction mixture containing 4 µg/ml of rhFurin, 50 µM substrate, 25 mM Tris, 1 mM CaCl2, 0.5% (w/v) Brij35, pH 9.0. The specific activity is >130 pmol/min/µg. |
| Endotoxin | < 1.000 Eu/µg |
| SwissProt/Accession | P09958 |
| Gene | FURIN |
| Residue | 108 to 715 |
| Sequence | DVYQEPTDPKFPQQWYLSGVTQRDLNVKAAWAQGYTGHGIVVSILDDGIE KNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVAN NGVCGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPE DDGKTVDGPARLAEEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDG YTNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQIVTTDLR QKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLN ANDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKCIIDILTE PKDIGKRLEVRKTVTACLGEPNHITRLEHAQARLTLSYNRRGDLAIHLVS PMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEA NNYGTLTKFTLVLYGTAPEGLPVPPESSGCKTLTSSQACVVCEEGFSLHQ KSCVQHCPPGFAPQVLDTHYSTENDVETIRASVCAPCHASCATCQGPALT DCLSCPSHASLDPVEQTCSRQSQSSRESPPQQQPPRLPPEVEAGQRLRAG LLPSHLPE |
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Product images
SDS-PAGE analysis of reduced ab167741 and staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 55-66 kDa.