All lanes : Anti-DNA Polymerase beta (mono methyl R151) antibody ( ab32542 ) at 1/250 dilution Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human DNA Polymerase beta (mono methyl R151) peptide (ab39687) at 1 µg/ml Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human DNA Polymerase beta (mono methyl R151) peptide (ab39687) at 1 µg/ml Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human DNA Polymerase beta (mono methyl R151) peptide (ab39687) at 1 µg/ml Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human DNA Polymerase beta peptide ( ab46818 ) at 1 µg/ml Lane 8 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate w