All lanes : Anti-Evx1 antibody ( ab15732 ) at 1 µg/ml Lane 1 : HeLa lysate Lane 2 : HeLa nuclear lysate Lane 3 : Jurkat lysate Lane 4 : A431 lysate Lane 5 : HEK293 lysate Lane 6 : HeLa lysate with Human Evx1 peptide (ab15743) at 1 µg/ml Lane 7 : HeLa nuclear lysate with Human Evx1 peptide (ab15743) at 1 µg/ml Lane 8 : Jurkat lysate with Human Evx1 peptide (ab15743) at 1 µg/ml Lane 9 : A431 lysate with Human Evx1 peptide (ab15743) at 1 µg/ml Lane 10 : HEK293 lysate with Human Evx1 peptide (ab15743) at 1 µg/ml Lysates/proteins at 20 µg per lane. Predicted band size : 42 kDa Observed band size : 42 kDa