Active human htrA1 full length protein

Name	Active human htrA1 full length protein
Supplier	Abcam
Catalog	ab134441
Category	Protein
Prices	$528.00
Sizes	25 µl
Applications	SDS-PAGE FA
Species Reactivities	Human
Nature	Recombinant
Source	Insect cells
Tag/Conjugation	His tag C-Terminus
Purity	> 70 % by SDS-PAGE. ab134441 was purified from insect cell culture supernatants.
Bioactivity	Proteolytic activity of ab134441 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 5 µg/ml ab134441 within 3 hours at 37°C.
SwissProt/Accession	Q92743
Gene	HTRA1
Residue	1 to 480
Sequence	Belongs to the peptidase S1B family. Contains 1 IGFBP N-terminal domain. Contains 1 Kazal-like domain. Contains 1 PDZ (DHR) domain.
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Product images

SDS-PAGE analysis of ab134441 (2&microg).

SDS-PAGE analysis of ab134441 (2µg).

Hydrolysis of ß-Casein by ab134441. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2 without or with ab134441 (5 µg/ml). After various time intervals aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.