Active human HtrA3 full length protein
| Name | Active human HtrA3 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab134450 |
| Category | Protein |
| Prices | $840.00 |
| Sizes | 50 µl |
| Applications | SDS-PAGE B/N FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Insect cells |
| Tag/Conjugation | His tag C-Terminus |
| Purity | > 70 % by SDS-PAGE. |
| Bioactivity | Proteolytic activity of ab134450 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 50 µg/ml HtrA3 within 2 hours at 37°C. |
| SwissProt/Accession | P83110 |
| Gene | HTRA3 |
| Residue | 1 to 453 |
| Sequence | Belongs to the peptidase S1B family. Contains 1 IGFBP N-terminal domain. Contains 1 Kazal-like domain. Contains 1 PDZ (DHR) domain. |
| Supplier Page | Shop |
Product images
SDS-PAGE analysis of ab134450 (1µg). Minor bands of HtrA3 fragments may be visible in the enzyme preparation.
Hydrolysis of beta-Casein by ab134450. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM CaCl 2 with or without HtrA3 (50 µg/ml). After indicated time intervals 10 µl aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.