All lanes : HIRA antibody ( ab20655 ) at 1 ug/ml Lane 1 : HeLa Nuclear Lysate Lane 2 : HeLa Nuclear Lysate with HIRA peptide (ab22456) at 1 ug/ml Lysates/proteins at 20 ug per lane. Predicted band size : 89kDa Observed band size : 89kDa Additional bands at : 32kDa (possible cross reactivity). ab20655 recognises a band at 89kDa, corresponding to the short isoform of HIRA (lane 1). This band is specifically blocked by the immunising peptide, ab22456 (lane 2). There is a band at 32kDa, which is not blocked by the immunising peptide and this is attributed to cross-reactivity.