Name |
Active human ITCH/AIP4 full length protein
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Supplier |
Abcam
|
Catalog |
ab190328
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Category |
Protein
|
Prices |
$572.00
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Sizes |
100 µg
|
Applications |
SDS-PAGE FA
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Species Reactivities |
Human
|
Nature |
Recombinant
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Source |
E. coli
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Purity |
>90% by SDS-PAGE.
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Bioactivity |
This untagged, ITCH/AIP4 isoform 2 recombinant protein demonstrates strong autoubiquitination activity in vitro. Typical enzyme concentration to support conjugation in vitro is 0.1-0.5 µM depending on experimental conditions.
|
SwissProt/Accession |
Q96J02
|
Gene |
ITCH
|
Residue |
1 to 862
|
Sequence |
MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQ SKKTEKCNNT NSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTA ALDIYETLKSNNMKLEEVVVTL QLGGDKEPTETIGDLSICLDGLQLES EVVTNGETTCSESASQNDDGSRSKDETRVSTNGS DDPEDAGAGENRRV SGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESD GS STGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWE QRVDQHGR VYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFT RTTTWQRPTLESVRNYEQWQ LQRSQLQGAMQQFNQRFIYGNQDLFATS QSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNT RITQWEDPRSQGQLNE KPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQ IAYV RDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRR RLWVIF PGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQ INPASYINPDHLKYFRFI GRFIAMALFHGKFIDTGFSLPFYKRILNKP VGLKDLESIDPEFYNSLIWVKENNIEECDL EMYFSVDKEILGEIKSHD LKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGF NEILPQ QYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVK EIDN EKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWL PRSHTCFNRLDLPPYK SYEQLKEKLLFAIEETEGFGQE
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