| Description |
The tenth fibronectin type III domain (10Fn3) that bound SUMO4 was isolated from the library using Phage display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=7 nM) for SUMO4 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
|