Active human KDM4B / JMJD2B protein fragment
| Name | Active human KDM4B / JMJD2B protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab198149 |
| Category | Protein |
| Prices | $1,281.00 |
| Sizes | 100 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | DDDDK tag C-Terminus |
| Purity | >= 41 % by SDS-PAGE. |
| Bioactivity | Specific Activity: 0.09 pmole/min/μg. Assay Conditions: 10µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and KDM4B / JMJD2B (20 – 100 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. |
| SwissProt/Accession | O94953 |
| Gene | KDM4B |
| Residue | 1 to 500 |
| Sequence | MGSEDHGAQNPSCKIMTFRPTMEEFKDFNKYVAYIESQGAHRAGLAKIIP PKEWKPRQTYDDIDDVVIPAPIQQVVTGQSGLFTQYNIQKKAMTVGEYRR LANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGSLYDDDVAQWN IGSLRTILDMVERECGTIIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINY LHFGEPKSWYAIPPEHGKRLERLAIGFFPGSSQGCDAFLRHKMTLISPII LKKYGIPFSRITQEAGEFMITFPYGYHAGFNHGFNCAESTNFATLRWIDY GKVATQCTCRKDMVKISMDVFVRILQPERYELWKQGKDLTVLDHTRPTAL TSPELSSWSASRASLKAKLLRRSHRKRSQPKKPKPEDPKFPGEGTAGAAL LEEAGGSVKEEAGPEVDPEEEEEEPQPLPHGREAEGAEEDGRGKLRPTKA KSERKKKSFGLLPPQLPPPPAHFPSEEALWLPSPLEPPVLGPGPAAMEES |
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