Active human KDM5C / Jarid1C / SMCX full length protein
| Name | Active human KDM5C / Jarid1C / SMCX full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab196392 |
| Category | Protein |
| Prices | $1,281.00 |
| Sizes | 20 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | His tag N-Terminus , DDDDK tag N-Terminus |
| Purity | >= 85 % by SDS-PAGE. |
| Bioactivity | Specific activity of 0.078 pmol/min/µg. |
| SwissProt/Accession | P41229 |
| Gene | KDM5C |
| Residue | 2 to 1560 |
| Sequence | EPGSDDFLPPPECPVFEPSWAEFRDPLGYIAKIRPIAEKSGICKIRPPAD WQPPFAVEV DNFRFTPRIQRLNELEAQTRVKLNYLDQIAKFWEIQGSS LKIPNVERRILDLYSLSKIVV EEGGYEAICKDRRWARVAQRLNYPPGK NIGSLLRSHYERIVYPYEMYQSGANLVQCNTRP FDNEEKDKEYKPHSI PLRQSVQPSKFNSYGRRAKRLQPDPEPTEEDIEKNPELKKLQIYG AGP KMMGLGLMAKDKTLRKKDKEGPECPPTVVVKEELGGDVKVESTSPKTFLE SKEELSH SPEPCTKMTMRLRRNHSNAQFIESYVCRMCSRGDEDDKLLL CDGCDDNYHIFCLLPPLPE IPKGVWRCPKCVMAECKRPPEAFGFEQAT REYTLQSFGEMADSFKADYFNMPVHMVPTEL VEKEFWRLVNSIEEDVT VEYGADIHSKEFGSGFPVSDSKRHLTPEEEEYATSGWNLNVMP VLEQS VLCHINADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWY GVPSL AAEHLEEVMKKLTPELFDSQPDLLHQLVTLMNPNTLMSHGVPV VRTNQCAGEFVITFPRA YHSGFNQGYNFAEAVNFCTADWLPAGRQCIE HYRRLRRYCVFSHEELICKMAACPEKLDL NLAAAVHKEMFIMVQEERR LRKALLEKGITEAEREAFELLPDDERQCIKCKTTCFLSALA CYDCPDG LVCLSHINDLCKCSSSRQYLRYRYTLDELPAMLHKLKVRAESFDTWANKV RVA LEVEDGRKRSLEELRALESEARERRFPNSELLQQLKNCLSEAEAC VSRALGLVSGQEAGP HRVAGLQMTLTELRAFLDQMNNLPCAMHQIGDV KGVLEQVEAYQAEAREALASLPSSPGL LQSLLERGRQLGVEVPEAQQL QRQVEQARWLDEVKRTLAPSARRGTLAVMRGLLVAGASV APSPAV |
| Supplier Page | Shop |
Product images
10 µL reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 50 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab196392 (50 – 200 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection.
10% SDS PAGE with Coomassie staining: 6 µg ab196392.