Active human KDM6B / JMJD3 protein fragment
| Name | Active human KDM6B / JMJD3 protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab196420 |
| Category | Protein |
| Prices | $1,281.00 |
| Sizes | 20 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | DDDDK tag C-Terminus |
| Purity | >= 50 % by SDS-PAGE. |
| Bioactivity | Specific activity: 0.022 pmole/min/μg. 10µl reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 150 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD3 (100 – 500 ng) added to the wells. Add antibody against demethylated K27 peptide. |
| SwissProt/Accession | O15054 |
| Gene | KDM6B |
| Residue | 1043 to 1643 |
| Sequence | KAKPPTAP APPSAPAPSA QPTPPSASVP GKKAREEAPG PPGVSRADML KLRSLSEGPP KELKIRLIKV ESGDKETFIA SEVEERRLRM ADLTISHCAA DVVRASRNAK VKGKFRESYL SPAQSVKPKI NTEEKLPREK LNPPTPSIYL ESKRDAFSPV LLQFCTDPRN PITVIRGLAG SLRLNLGLFS TKTLVEASGE HTVEVRTQVQ QPSDENWDLT GTRQIWPCES SRSHTTIAKY AQYQASSFQE SLQEEKESED EESEEPDSTT GTPPSSAPDP KNHHIIKFGT NIDLSDAKRW KPQLQELLKL PAFMRVTSTG NMLSHVGHTI LGMNTVQLYM KVPGSRTPGH QENNNFCSVN INIGPGDCEW FAVHEHYWET ISAFCDRHGV DYLTGSWWPI LDDLYASNIP VYRFVQRPGD LVWINAGTVH WVQATGWCNN IAWNVGPLTA YQYQLALERY EWNEVKNVKS IVPMIHVSWN VARTVKISDP DLFKMIKFCL LQSMKHCQVQ RESLVRAGKK IAYQGRVKDE PAYYCNECDV EVFNILFVTS ENGSRNTYLV HCEGCARRRS AGLQGVVVLE QYRTEELAQA YDAFTLVRAR RARGQRRRAL GQAAGTGFGS PAAPFPEPPP AFSPQAPAST SR |
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