Active human KMT1A / SUV39H1 full length protein
| Name | Active human KMT1A / SUV39H1 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab196397 |
| Category | Protein |
| Prices | $858.00 |
| Sizes | 5 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | >= 5 % by SDS-PAGE. |
| Bioactivity | Specific Activity: 0.4 pmol/min/μg. Assay conditions: 80 µL containing 50 mM Tris pH 8.8, 5 mM MgCl 2 , 4 mM DTT, 10 µM substrate peptide, 20 µM S-adenosylmethionine, and 20-40 ng ab196397 for 1 hour at room temperature. Add antibody against methylated K9 residue of histone H3, incubate 1 hour. Then, add secondary HRP-labeled antibody and incubate 30 minutes. Finally, add HRP chemiluminescent substrates and read luminescence. |
| SwissProt/Accession | O43463 |
| Gene | SUV39H1 |
| Residue | 2 to 412 |
| Sequence | AENLKGCSVCCKSSWNQLQDLCRLAKLSCPALGISKRNLYDFEVEYLCDY KKIREQEYYLVKWRGYPDSESTWEPRQNLKCVRILKQFHKDLERELLRRH HRSKTPRHLDPSLANYLVQKAKQRRALRRWEQELNAKRSHLGRITVENEV DLDGPPRAFVYINEYRVGEGITLNQVAVGCECQDCLWAPTGGCCPGASLH KFAYNDQGQVRLRAGLPIYECNSRCRCGYDCPNRVVQKGIRYDLCIFRTD DGRGWGVRTLEKIRKNSFVMEYVGEIITSEEAERRGQIYDRQGATYLFDL DYVEDVYTVDAAYYGNISHFVNHSCDPNLQVYNVFIDNLDERLPRIAFFA TRTIRAGEELTFDYNMQVDPVDMESTRMDSNFGLAGLPGSPKKRVRIECK CGTESCRKYLF |
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