Recombinant JARID1B / KDM5B (2-751) was run on a 12. 5% SDS-PAGE gel and stained with Coomassie Blue. MW: 100 kDa Purity: >80%
HTRF assay for JARID1B /KDM5B (2-751) activity 3 µM H3K4me3 (1-21 aa) peptide was incubated with KDM5B (2-751) protein in reaction buffer for 1 hour at room temperature. Anti-H3K4me2-d2 antibody was used to detect reaction product.
MALDI-TOF for JARID1B / KDM5B (2-751) activity. 3. 3 µM H3K4me3 (1-21 aa) peptide was incubated with 100 nM or 200 nM JARID1B / KDM5B (2-751) protein in reaction buffer for 2 hours at room temperature. MALDI-TOF was used for detection. Catalytic Ability: > 60 turnovers/ enzyme molecule