Recombinant KDM7A protein gel. KDM7A protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
HTRF assay for KDM7A activity. 1 µM H3K9me2 peptide was incubated with KDM7A protein in reaction buffer with or without 0. 02% Triton X-100, respectively, for 1 hour at room temperature. KDM7A enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me0) was measured using H3K9me0-specific antibody. The results show that 0. 02% Triton X-100 slightly inhibits the activity of KDM7A.
HTRF assay for KDM7A activity. 1 µM H3K9me2 peptide was incubated with KDM7A protein in reaction buffer with or without 0. 02% Triton X-100, respectively, for 1 hour at room temperature. KDM7A enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me0) was measured using H3K9me0-specific antibody. The results show that 0. 02% Triton X-100 slightly inhibits the activity of KDM7A.