All lanes : Anti-hUPF2/RENT2 antibody ( ab28712 ) at 1/250 dilution Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human hUPF2/RENT2 peptide (ab32332) at 1 µg/ml Lysates/proteins at 15 µg per lane. Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution Performed under reducing conditions. Predicted band size : 147 kDa Observed band size : 153 kDa ( why is the actual band size different from the predicted? ) Additional bands at : 51 kDa. We are unsure as to the identity of these extra bands.