Active dog PDE4B full length protein
| Name | Active dog PDE4B full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198425 |
| Category | Protein |
| Prices | $1,250.00 |
| Sizes | 5 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Dog |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | >90% by SDS-PAGE. |
| Bioactivity | Specific Activity: ≥ 5665 pmol/min/µg Unit Definition: One unit is defined as the amount of ab198425 that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C. Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl 2 , 0.1 mg/ml BSA, 0.05% Tween-20, 200µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198425 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. |
| SwissProt/Accession | Q07343 |
| Gene | PDE4B |
| Residue | 2 to 679 |
| Sequence | MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHLVPRGSGSGIQSIIMKPRSRSTSSLRTTEAACFDV DNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKS MSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRS PMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMAS NKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKE KKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWG LHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHY HADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVD HPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLT KKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNY SDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGME ISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNR EWYQSTIPQSPSPAPDEQEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQ VEEDTSCSDSKTLCTQDSESTEIPLDEQVEEETVGEEENSQPEACVIEDH SPDT |
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