Name | Active human LDL Receptor protein fragment |
---|---|
Supplier | Abcam |
Catalog | ab198760 |
Category | Protein |
Prices | $1,281.00 |
Sizes | 50 µg |
Applications | SDS-PAGE FA |
Species Reactivities | Human |
Nature | Recombinant |
Source | HEK 293 cells |
Tag/Conjugation | DDDDK tag C-Terminus |
Purity | >= 87 % by SDS-PAGE. |
Bioactivity | Assay Conditions: 50 µL reaction mix (50 mM HEPES pH 7.4, 100 mM NaCl, 200 µM CaCl 2 , 0.05% Tween-20, 0.1% BSA, and up to 1500 ng PCSK9) is added to the wells coated with ab198760. Incubate for 2hr. Add antibody against polyhistidine and then incubate 1 hr. Add secondary HRP labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. |
SwissProt/Accession | P01130 |
Gene | LDLR |
Residue | 22 to 788 |
Sequence | AVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSG DFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKC ISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDP DCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPD CKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCV NVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDN NGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNL EGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTS LIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISR DIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRA IVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLL SGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWT DIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTT LSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVAT QETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQA LGDVAGRGNEKKPSSVRDYKDDDDK |
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