Active human LRRK2 (mutated G2019 S) protein fragment
| Name | Active human LRRK2 (mutated G2019 S) protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab196124 |
| Category | Protein |
| Prices | $648.00 |
| Sizes | 10 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | > 3 % by SDS-PAGE. |
| Bioactivity | Specific Activity: 116 pmol/min/µg |
| SwissProt/Accession | Q5S007 |
| Gene | LRRK2 |
| Residue | 960 to 2527 |
| Sequence | RSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVH LEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSC IANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKL EQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFS ARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQ YLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKE IPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFK HIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKK SDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFM TQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDE KQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESL NFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRE NQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQ ILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIA LPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLI NRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHP ESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLK KWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDL ILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFN KHTSLRLLRQELVVLCHLHHPSLISLLAAGI |
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Product images
4-20% SDS-PAGE analysis of ab196124 with Coomassie staining Lane 1: 0.4 µg active Human LRRK2 (mutated G2019 S) protein fragment (ab196124) Lane 2: Protein marker
Activity of ab196124. Reaction buffer: 10 mM Tris, pH 7.4, 5 mM MgCl2, 0.5 mM EDTA, 0.5 mM DTT, 200 µM ATP, 200 µg LRRKtide for 1 hour at room temperature.