All lanes : Anti-LAMP2b antibody - Lysosome Marker ( ab18529 ) at 1 µg/ml Lane 1 : Human skeletal muscle lysate Lane 2 : Human liver lysate Lane 3 : Human skeletal muscle lysate with Human LAMP2b peptide (ab23321) at 1 µg/ml Lane 4 : Human liver lysate with Human LAMP2b peptide (ab23321) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution Predicted band size : 100 kDa Observed band size : 45 kDa ( why is the actual band size different from the predicted? )