Name |
Active human NDRG1 full length protein
|
Supplier |
Abcam
|
Catalog |
ab87685
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Category |
Protein
|
Prices |
$192.00, $588.00
|
Sizes |
100 µg, 500 µg
|
Applications |
SDS-PAGE
|
Species Reactivities |
Human
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Nature |
Recombinant
|
Source |
E. coli
|
Purity |
> 95 % by SDS-PAGE. ab87685 is purified using conventional chromatography.
|
Bioactivity |
The ED50 for this effect is 0.5 - 1.5 ng/ml. Measured in a cell proliferation assay using MCF7 cell. Activity Assay Cell line: MCF7 (Human breast adenocarcinoma cell) Maintenance Condition: RPMI 1640 containing 10% FBS Assay medium: serum free RPMI 1640 Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul) Incubation time : 40 hrs (after sample treatment) Concentration range: 0.19ng/ml - 50ng/ml Detection method : BrdU assay
|
SwissProt/Accession |
Q92597
|
Gene |
NDRG1
|
Sequence |
MSREMQDVDL AEVKPLVEKG ETITGLLQEF DVQEQDIETL HGSVHVTLCG TPKGNRPVIL TYHDIGMNHK TCYNPLFNYE DMQEITQHFA VCHVDAPGQQ DGAASFPAGY MYPSMDQLAE MLPGVLQQFG LKSIIGMGTG AGAYILTRFA LNNPEMVEGL VLINVNPCAE GWMDWAASKI SGWTQALPDM VVSHLFGKEE MQSNVEVVHT YRQHIVNDMN PGNLHLFINA YNSRRDLEIE RPMPGTHTVT LQCPALLVVG DSSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLDGT RSRSHTSEGT RSRSHTSEGT RSRSHTSEGA HLDITPNSGA AGNSAGPKSM EVSCLEHHHH HH
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