Active human P4HB full length protein

• Supplier: Abcam
• Catalog: ab73280
• Category: Protein
• Prices: $192.00
• Sizes: 100 µg
• Applications: FA SDS-PAGE
• Species Reactivities: Human
• Nature: Recombinant
• Source: E. coli
• Purity: > 95 % by SDS-PAGE. This protein was purified by proprietary chromatographic techniques. Purity greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Bioactivity: Reductase Activity: 1.0 x 10 -3 OD650nm/ min -2 . By measuring the turbidity increase at 650 nm due to insulin reduction (Holmgren, A. (1979) J. Biol. Chem. 254, 9627–9632). The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time (Mart´nez-Galisteo, E., Padilla, C. A., Garcia-Alfonso, C., Lo´pez-Barea, J. and Barcena, J. A. (1993) Biochimie (Paris) 75, 803–809). Isomerase Activity: 0.5 µmol active RNase A min -1 µmol P4HB -1 . According to the re-activation of reduced and denatured RNase A (Lyles, M. M. and Gilbert, H. F. (1991) Biochemistry 30, 613-619).
• SwissProt/Accession: P07237
• Gene: P4HB
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala.