Active human PADI4 / PAD4 full length protein
| Name | Active human PADI4 / PAD4 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab196393 |
| Category | Protein |
| Prices | $1,250.00 |
| Sizes | 10 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | DDDDK tag C-Terminus |
| Purity | >= 65 % by SDS-PAGE. |
| Bioactivity | Specific activity of ≥80 pmole/min/µg. |
| SwissProt/Accession | Q9UM07 |
| Gene | PADI4 |
| Residue | 1 to 663 |
| Sequence | MAQGTLIRVTPEQPTHAVCVLGTLTQLDICSSAPEDCTSFSINASPGVVV DIAHGPPAKK KSTGSSTWPLDPGVEVTLTMKVASGSTGDQKVQISYYG PKTPPVKALLYLTGVEISLCAD ITRTGKVKPTRAVKDQRTWTWGPCGQ GAILLVNCDRDNLESSAMDCEDDEVLDSEDLQDM SLMTLSTKTPKDFF TNHTLVLHVARSEMDKVRVFQATRGKLSSKCSVVLGPKWPSHYLMV PG GKHNMDFYVEALAFPDTDFPGLITLTISLLDTSNLELPEAVVFQDSVVFR VAPWIMTP NTQPPQEVYACSIFENEDFLKSVTTLAMKAKCKLTICPEE ENMDDQWMQDEMEIGYIQAP HKTLPVVFDSPRNRGLKEFPIKRVMGPD FGYVTRGPQTGGISGLDSFGNLEVSPPVTVRG KEYPLGRILFGDSCYP SNDSRQMHQALQDFLSAQQVQAPVKLYSDWLSVGHVDEFLSFVP APDR KGFRLLLASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQKIKNILSNKT LREHNS FVERCIDWNRELLKRELGLAESDIIDIPQLFKLKEFSKAEAF FPNMVNMLVLGKHLGIPK PFGPVINGRCCLEEKVCSLLEPLGLQCTFI NDFFTYHIRHGEVHCGTNVRRKPFSFKWWN MVP |
| Supplier Page | Shop |
Product images
Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl 2 , and 2 mM DTT) containing ab196393 and 10 mM BAEE as a substrate for 25 min at 37°C. Amount of ammonia produced was measured and used for specific activity calculation.
10% SDS PAGE with Coomassie staining: 2.4 µg ab196393.