| Bioactivity |
Specific Activity: 32638 pmoles/min/µg. Analysis of enzymatic activity was performed according to Zlyte assay protocol: Different concentration of PAK1 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR14) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. Fluorescence was then detected using Lambda exc=460±40 nm and Lambda em=528±20 nm filters.
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