Active human PAK6 protein fragment

Name Active human PAK6 protein fragment
Supplier Abcam
Catalog ab95321
Category Protein
Prices $761.00
Sizes 10 µg
Applications FA
Species Reactivities Human
Nature Recombinant
Source E. coli
Purity > 90 % by SDS-PAGE.
Bioactivity Specific Activity: 2.1 nmoles/min/µg. Analysis of enzymatic activity: 1. Different concentration of PAK6 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 20) at room temperature for 1 hour. 2. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. 3. Fluorescence was detected using ?exc=460±40 nm and ?em=528±20 nm filters.
SwissProt/Accession Q9NQU5
Gene PAK6
Residue 385 to 680
Sequence Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain.
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