Active human PCSK9 full length protein
| Name | Active human PCSK9 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198471 |
| Category | Protein |
| Prices | $1,281.00 |
| Sizes | 50 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Tag/Conjugation | His tag C-Terminus |
| Bioactivity | 50 µL reaction mix (50 mM HEPES pH 7.4, 100 mM NaCl, 200 µM CaCl 2 , 0.05% Tween-20, 0.1% BSA, and up to 1500 ng PCSK9) is added to wells precoated with LDLR Ectodomain. Incubate for 2 hour. Add antibody against polyhistidine and then incubate 1 hour. Add secondary HRP labeled antibody and incubate 30 minutes. Finally, add HRP chemiluminescent substrates and read luminescence. |
| SwissProt/Accession | Q8NBP7 |
| Gene | PCSK9 |
| Residue | 31 to 692 |
| Sequence | QEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVV VLKEETHL SQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSG DLLELALKLPHVDYIEEDS SVFAQSIPWNLERITPPRYRADEYQPPDG GSLVEVYLLDTSIQSDHREIEGRVMVTDFE NVPEEDGTRFHRQASKCD SHGTHLAGVVSGRDAGVAKGASMRSLRVLNCQGKGTV SGTLIGLEFIR KSQLVQPVGPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFR D DACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIG ASSDCST CFVSQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHF SAKDVINEAWFPEDQR VLTPNLVAALPPSTHGAGWQLFCRTVWSAHSG PTRMATAVARCAPDEELLSCSSFS RSGKRRGERMEAQGGKLVCRAHNA FGGEGVYAIARCCLLPQANCSVHTAPPAEAS MGTRVHCHQQGHVLTGC SSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASC CHAPGLECKV KEHGIPAPQEQVTVACEEGWTLTGCSALPGTSHVLGAYAVDNTCVV RS RDVSTTGSTSEGAVTAVAICCRSRHLAQASQELQHHHHHH |
| Supplier Page | Shop |
