Active human PDE10A full length protein

Name Active human PDE10A full length protein
Supplier Abcam
Catalog ab198428
Category Protein
Prices $1,250.00
Sizes 10 µg
Applications FA SDS-PAGE
Species Reactivities Human
Nature Recombinant
Source Baculovirus infected sf9 cells
Tag/Conjugation GST tag N-Terminus
Purity >= 48 % by SDS-PAGE.
Bioactivity Specific Activity: 3091 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmole of 3',5'-cAMP to 5'- AMP per min at 37°C. Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl 2 , 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and 0.25-4 ng/µl PDE10A at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
SwissProt/Accession Q9Y233
Gene PDE10A
Residue 2 to 789
Sequence EDGPSNNASCFRRLTECFLSPSLTDEKVKAYLSLHPQVLDEFVSESVSAE TVEKWLKRKNNKSEDESAPKEVSRYQDTNMQGVVYELNSYIEQRLDTGGD NQLLLYELSSIIKIATKADGFALYFLGECNNSLCIFTPPGIKEGKPRLIP AGPITQGTTVSAYVAKSRKTLLVEDILGDERFPRGTGLESGTRIQSVLCL PIVTAIGDLIGILELYRHWGKEAFCLSHQEVATANLAWASVAIHQVQVCR GLAKQTELNDFLLDVSKTYFDNIVAIDSLLEHIMIYAKNLVNADRCALFQ VDHKNKELYSDLFDIGEEKEGKPVFKKTKEIRFSIEKGIAGQVARTGEVL NIPDAYADPRFNREVDLYTGYTTRNILCMPIVSRGSVIGVVQMVNKISGS AFSKTDENNFKMFAVFCALALHCANMYHRIRHSECIYRVTMEKLSYHSIC TSEEWQGLMQFTLPVRLCKEIELFHFDIGPFENMWPGIFVYMVHRSCGTS CFELEKLCRFIMSVKKNYRRVPYHNWKHAVTVAHCMYAI LQNNHTLFT DLERKGLLIACLCHDLDHRGFSNSYLQKFDHPLAALYSTSTMEQHHFSQT VSILQLEGHNIFSTLSSSEYEQVLEIIRKAIIATDLALYFGNRKQLEEMY QTGSLNLNNQSHRDRVIGLMMTACDLCSVTKLWPVTKLTANDIYAEFWAE GDEMKKLGIQPIPMMDRDKKDEVPQGQLGFYNAVAIPCYTTLTQILPPTE PLLKACRDNLSQWEKVIRGEETATWISSPSVAQKAAASED
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