Name | Active human PDE10A full length protein |
---|---|
Supplier | Abcam |
Catalog | ab198428 |
Category | Protein |
Prices | $1,250.00 |
Sizes | 10 µg |
Applications | FA SDS-PAGE |
Species Reactivities | Human |
Nature | Recombinant |
Source | Baculovirus infected sf9 cells |
Tag/Conjugation | GST tag N-Terminus |
Purity | >= 48 % by SDS-PAGE. |
Bioactivity | Specific Activity: 3091 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmole of 3',5'-cAMP to 5'- AMP per min at 37°C. Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl 2 , 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and 0.25-4 ng/µl PDE10A at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. |
SwissProt/Accession | Q9Y233 |
Gene | PDE10A |
Residue | 2 to 789 |
Sequence | EDGPSNNASCFRRLTECFLSPSLTDEKVKAYLSLHPQVLDEFVSESVSAE TVEKWLKRKNNKSEDESAPKEVSRYQDTNMQGVVYELNSYIEQRLDTGGD NQLLLYELSSIIKIATKADGFALYFLGECNNSLCIFTPPGIKEGKPRLIP AGPITQGTTVSAYVAKSRKTLLVEDILGDERFPRGTGLESGTRIQSVLCL PIVTAIGDLIGILELYRHWGKEAFCLSHQEVATANLAWASVAIHQVQVCR GLAKQTELNDFLLDVSKTYFDNIVAIDSLLEHIMIYAKNLVNADRCALFQ VDHKNKELYSDLFDIGEEKEGKPVFKKTKEIRFSIEKGIAGQVARTGEVL NIPDAYADPRFNREVDLYTGYTTRNILCMPIVSRGSVIGVVQMVNKISGS AFSKTDENNFKMFAVFCALALHCANMYHRIRHSECIYRVTMEKLSYHSIC TSEEWQGLMQFTLPVRLCKEIELFHFDIGPFENMWPGIFVYMVHRSCGTS CFELEKLCRFIMSVKKNYRRVPYHNWKHAVTVAHCMYAI LQNNHTLFT DLERKGLLIACLCHDLDHRGFSNSYLQKFDHPLAALYSTSTMEQHHFSQT VSILQLEGHNIFSTLSSSEYEQVLEIIRKAIIATDLALYFGNRKQLEEMY QTGSLNLNNQSHRDRVIGLMMTACDLCSVTKLWPVTKLTANDIYAEFWAE GDEMKKLGIQPIPMMDRDKKDEVPQGQLGFYNAVAIPCYTTLTQILPPTE PLLKACRDNLSQWEKVIRGEETATWISSPSVAQKAAASED |
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