Active human PDE4A full length protein
| Name | Active human PDE4A full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198466 |
| Category | Protein |
| Prices | $1,250.00 |
| Sizes | 10 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | >= 20 % by SDS-PAGE. |
| Bioactivity | Specific Activity: ≥ 4,400 pmol/min/µg One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl 2 , 1 mM MnCl 2 , 200 µM cAMP, and 0.2 ng/µl ab198466 at 37ºC for 20 min. Quantified by 5′-nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. |
| SwissProt/Accession | P27815 |
| Gene | PDE4A |
| Residue | 2 to 825 |
| Sequence | RSGAAPRARPRPPALALPPTGPESLTHFPFSDEDTRRHPPGRSVSFEAEN GPTPSPGRSPLDSQASPGLVLHAGAATSQRRESFLYRSDSDYDMSPKTMS RNSSVTSEAHAEDLIVTPFAQVLASLRSVRSNFSLLTNVPVPSNKRSPLG GPTPVCKATLSEETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKF KRMLNRELTHLSEMSRSGNQVSEYISTTFLDKQNEVEIPSPTMKEREKQQ APRPRPSQPPPPPVPHLQPMSQITGLKKLMHSNSLNNSNIPRFGVKTDQE ELLAQELENLNKWGLNIFCVSDYAGGRSLTCIMYMIFQERDLLKKFRIPV DTMVTYMLTLEDHYHADVAYHNSLHAADVLQSTHVLLATPALDAVFTDLE ILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFK LLQEDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVET KKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAE FFQQGDRERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVH PDAQEILDTLEDNRDWYYSAIRQSPSPPPEEESRGPGHPPLPDKFQFELT LEEEEEEEI SMAQIPCTAQEALTAQGLSGVEEALDATIAWEASPAQES LEVMAQEASLEAELEAVYLTQQAQSTGSAPVAPDEFSSREEFVVAVSHSS PSALALQSPLLPAWRTLSVSEHAPGLPGLPSTAAEVEAQREHQAAKRACS ACAGTFGEDTSALPAPGGGGSGGDPT |
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