Active human PDE4D full length protein
| Name | Active human PDE4D full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198456 |
| Category | Protein |
| Prices | $1,250.00 |
| Sizes | 5 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | >95% by SDS-PAGE . |
| Bioactivity | Specific Activity: ≥ 13,600 pmol/min/µg Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl 2 , 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198456 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. |
| SwissProt/Accession | Q08499 |
| Gene | PDE4D |
| Residue | 2 to 507 |
| Sequence | ASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPTQKE KEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNK WGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLED HYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHD VDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQN LTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLD NYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERG MEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLED NREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSG SQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVID DRSPDT |
| Supplier Page | Shop |
