Active human Peroxiredoxin 1 full length protein
| Name | Active human Peroxiredoxin 1 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab79945 |
| Category | Protein |
| Prices | $302.00 |
| Sizes | 50 µg |
| Applications | WB FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus |
| Purity | > 90 % by SDS-PAGE. |
| Bioactivity | Specific Activity between 800 and 1000 pmol/min/µg. Please enquire as to activity of specific lots. 1.88 µg of Peroxiredoxin 1 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H 2 O 2 . NADPH oxidation was monitored (fluorescence decrease) for the next 10 min. |
| SwissProt/Accession | Q06830 |
| Gene | PRDX1 |
| Sequence | Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain. |
| Supplier Page | Shop |
Product images
Lane 1: ab79945 on 14% SDS-PAGE, Coomassie staining, 20µg. Lane 2: Protein marker.
Anti-Peroxiredoxin 1 antibody ( ab41906 ) at 1 µg/ml + Active human Peroxiredoxin 1 full length protein (ab79945) at 0.01 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080 ) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Exposure time : 30 seconds