| Name |
Active human PIN4 full length protein
|
| Supplier |
Abcam
|
| Catalog |
ab105618
|
| Category |
Protein
|
| Prices |
$308.00, $1,234.00
|
| Sizes |
100 µg, 500 µg
|
| Applications |
FA SDS-PAGE MS
|
| Species Reactivities |
Human
|
| Nature |
Recombinant
|
| Source |
E. coli
|
| Tag/Conjugation |
His tag N-Terminus
|
| Purity |
> 85 % by SDS-PAGE. ab105618 was purified using conventional chromatography.
|
| Bioactivity |
Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. Add 10 µl of recombinant PIN4 protein with 1 µg in assay buffer. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) Record the increase in A405 nm for 30 minutes at 25°C.
|
| SwissProt/Accession |
Q9Y237
|
| Gene |
PIN4
|
| Residue |
1 to 156
|
| Sequence |
MGSSHHHHHHSSGLVPRGSH MPMAGLLKGLVRQLERFSVQQQASKMPPKG KSGSGKAGKGGAASGSDSADKKAQGPKGGGNAVKVRHILCEKHGKIMEAM EKLKSGMRFNEVAAQYSEDKARQGGDLGWMTRGSMVGPFQEAAFALPVSG MDKPVFTDPPVKTKFGYHIIMVEGRK
|
| Supplier Page |
Shop
|
15% SDS-PAGE analysis of 3µg ab105618.