All lanes : Anti-p53 (phospho S315) antibody ( ab1647 ) at 1 µg/ml Lane 1 : Human Embryonic Stem Cell Lysate Lane 2 : Hela lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate ( ab7899 ) Lane 4 : Human Embryonic Stem Cell Lysate with Human p53 (phospho S315) peptide (ab28896) at 1 µg/ml Lane 5 : Hela lysate with Human p53 (phospho S315) peptide (ab28896) at 1 µg/ml Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate ( ab7899 ) with Human p53 (phospho S315) peptide (ab28896) at 1 µg/ml Lane 7 : Human Embryonic Stem Cell Lysate with p53 (unmodified ) peptide ( ab28897 ) at 1 µg/ml Lane 8 : Hela lysate with p53 (unmodified ) peptide ( ab28897 ) at 1 µg/ml Lane 9 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate ( ab7899 ) with p53 (unmodified ) peptide ( ab28897 ) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Rabbit IgG secondary antibody ( ab28446 ) at 1/10000 dilution Performed under reducing conditions.