NGF Receptor (209 aa) - Fc Chimera
| Name | NGF Receptor (209 aa) - Fc Chimera |
|---|---|
| Supplier | Abcam |
| Catalog | ab83679 |
| Applications | SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Purity | > 95 % by SDS-PAGE. |
| SwissProt/Accession | P08138 |
| Gene | NGFR |
| Sequence | KEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVS ATEPCKPC TECVGLQSMSAPCVEADDAVCRCAYGYYQDETTGRCEACR VCEAGSGLVFSCQDKQN TVCEECPDGTYSDEANHVDPCLPCTVCEDTE RQLRECTRWADAECEEIPGRWITRSTP PEGSDSTAPSTQEPEAPPEQD LIASTVAGVVTTVMGIPKVDKKVEPKSCDKTHTCPPCP APELLGGPSV FLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKT KPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCRVSNKALPAPIEKTISK AKGQPREPQV YTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQ PENNYKTTPPVLDSDGSFFLY SKLTVDKSRWQQGNVFSCSVMHEALHN HYTQKSLSLSPGK |
| Supplier Page | Shop |
Product images
Densitometry of protein isoforms visualised by 2-D. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83679 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: MW markers; Lane 2: ab83679; Lane 3: ab83679 treated with PNGase F to remove potential N-linked glycans; Lane 4: ab83679 treated with a glycosidase cocktail to remove potential N and O-linked glycans. 10 μg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW after treatment with glycosidase cocktail indicates the presence of Olinked glycans. Faint bands in lane 3 and lane 4 are glycosidase enzymes.
A sample of ab83679 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein loaded per lane; Deep Purple™ stained.