All lanes : Anti-PACT (PKR activating protein) / PRKRA antibody ( ab31967 ) at 1 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human PACT (PKR activating protein) / PRKRA peptide (ab30768) at 1 µg/ml Lysates/proteins at 10 µg per lane. Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution Performed under reducing conditions. Predicted band size : 34 kDa Observed band size : 34 kDa Additional bands at : 60 kDa (possible cross reactivity, but this band is not blocked).