All lanes : Anti-pan Cadherin antibody - Plasma Membrane Marker ( ab16505 ) at 1 µg/ml Lane 1 : Mouse heart Lane 2 : HeLa cell lysate Lane 3 : 3T3 cell lysate Lane 4 : Mouse muscle Lane 5 : Human heart Lane 6 : Mouse heart with Human pan Cadherin peptide (ab17098) at 1 µg/ml Lane 7 : HeLa cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/ml Lane 8 : 3T3 cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/ml Lane 9 : Mouse muscle with Human pan Cadherin peptide (ab17098) at 1 µg/ml Lane 10 : Human heart with Human pan Cadherin peptide (ab17098) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Goat anti-rabbit conjugated to Alexafluor 680 at 1/10000 dilution Performed under reducing conditions. Predicted band size : 100 kDa Observed band size : 135 kDa ( why is the actual band size different from the predicted? )