| Name |
Active human PRMT1 full length protein
|
| Supplier |
Abcam
|
| Catalog |
ab198122
|
| Category |
Protein
|
| Prices |
$1,250.00
|
| Sizes |
20 µg
|
| Applications |
FA SDS-PAGE
|
| Species Reactivities |
Human
|
| Nature |
Recombinant
|
| Source |
Baculovirus infected sf9 cells
|
| Tag/Conjugation |
GST tag N-Terminus
|
| Purity |
>= 68 % by SDS-PAGE.
|
| Bioactivity |
Specific Activity: 6.2 pmol/min/µg. Assay Conditions: 50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 1-5 ng ab198122 add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
|
| SwissProt/Accession |
Q99873
|
| Gene |
PRMT1
|
| Residue |
2 to 371
|
| Sequence |
AAAEAANCIMENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKD YYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGI LCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVEL PVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVT AIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACL IKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGF STSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTI DLDFKGQLCELSCSTDYRMR
|
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