Western blot for ab12107 - Rabbit polyclonal to Pin1 (1/500) Secondary ab: ab6721 goat polyclonal to Rabbit IgG HRP (1/5000) Pin1 blocking peptide = ab12397 (1 µ g/ml) WB exposure time: 2 minutes Expected molecular weight: 18.4kDa; Observed band: ~20kDa Lane 1: 20 µ g HeLa Nuclear Extract Lane 2: 20 µ g HeLa Whole Cell Lysate Lane 3: 20 µ g A431 Whole Cell Lysate Lane 4: 20 µ g Jurkat Whole Cell Lysate Lane 5: 20 µ g 293 Whole Cell Lysate Lane 6: 20 µ g HeLa Nuclear Extract + blocking peptide Lane 7: 20 µ g HeLa Whole Cell Lysate + blocking peptide Lane 8: 20 µ g A431 Whole Cell Lysate + blocking peptide Lane 9: 20 µ g Jurkat Whole Cell Lysate + blocking peptide Lane 10: 20 µ g 293 Whole Cell Lysate + blocking peptide NB: The Pin1 specific band (~20kDa) is completely blocked by the ab12397 peptide. A seperate band at ~23kDa band is detected by ab12107 that is not blocked in these tests; the identity of this band is not known.