Densitometry of protein isoforms visualised by 2-DE. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab84148 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: ab84148 Lane 2: ab84148 treated with PNGase F to remove potential N-linked glycans Lane 3: ab84148 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10µg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.