Name | Active human SIRT2 full length protein |
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Supplier | Abcam |
Catalog | ab138791 |
Category | Protein |
Prices | $239.00 |
Sizes | 20 µg |
Applications | SDS-PAGE FA |
Species Reactivities | Human |
Nature | Recombinant |
Source | E. coli |
Purity | >90% by SDS-PAGE. |
Bioactivity | SPECIFIC ACTIVITY: = 80 units/mg. Activity of ab138791 was tested using the substrate Arg-His-Lys-Lys(Ac)-AFC, which is a fluorogenic, acetylated peptide based on residues 379-382 of p53. UNIT DEFINITION: One unit of the recombinant SIRT2 is defined as the amount of enzyme that deacetylates 1 pmol of substrate Arg-His-Lys-Lys(Ac)-AFC per minute at 37°C in a reaction solution containing 50 mM Tris, pH 7.5, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl 2 , 0.1 mM NAD + and 1 mg/ml BSA. |
SwissProt/Accession | Q8IXJ6 |
Gene | SIRT2 |
Residue | 1 to 389 |
Sequence | MAEPDPSHPL ETQAGKVQEA QDSDSDSEGG AAGGEADMDF LRNLFSQTLS LGSQKERLLD ELTLEGVARY MQSERCRRVI CLVGAGISTS AGIPDFRSPS TGLYDNLEKY HLPYPEAIFE ISYFKKHPEP FFALAKELYP GQFKPTICHY FMRLLKDKGL LLRCYTQNID TLERIAGLEQ EDLVEAHGTF YTSHCVSASC RHEYPLSWMK EKIFSEVTPK CEDCQSLVKP DIVFFGESLP ARFFSCMQSD FLKVDLLLVM GTSLQVQPFA SLISKAPLST PRLLINKEKA GQSDPFLGMI MGLGGGMDFD SKKAYRDVAW LGECDQGCLA LAELLGWKKE LEDLVRREHA SIDAQSGAGV PNPSTSASPK KSPPPAKDEA RTTEREKPQ |
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