| Bioactivity |
One unit is defined as the amount of enzyme required to produce 1 nmole of 7-amino-4-methylcouarin per minute at 25°C in 50mM Tris-HCL, pH8.0, 137 mM NaCl, 2.7mM KCl, 1mM MgCl2 , containing 125 µM p53 amino acids 217-320, and 6mM NAD. One unit is defined as the amount of enzyme required to produce 1 nmole of 7-amino-4-methylcouarin per minute at 25°C in 50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl 2 , containing 125 µM p53 amino acids 317-320 (Gln-Pro-Lys-Lys(e-acetyl)-AMC), and 6mM NAD + Specific activity: batch-specific.
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