Active human SMURF1 protein fragment
| Name | Active human SMURF1 protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab95928 |
| Category | Protein |
| Prices | $396.00 |
| Sizes | 20 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus |
| Tag/Conjugation | DDDDK tag N-Terminus |
| Purity | 80 % . Purity is >80% by SDS-PAGE. |
| Bioactivity | Specific Activity: 0.0055 pmol/min/µg Assay conditions: 50 µl reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and SMURF1 (0-1 µg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against SMURF1, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm). |
| SwissProt/Accession | Q9HCE7 |
| Gene | SMURF1 |
| Residue | 150 to 757 |
| Sequence | Contains 1 C2 domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 2 WW domains. |
| Supplier Page | Shop |
