All lanes : Anti-PTP lambda antibody ( ab13223 ) at 1/500 dilution Lane 1 : Human brain lysate Lane 2 : Human kidney lysate Lane 3 : Human pancreas lysate Lane 4 : Human heart lysate Lane 5 : Human brain lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 6 : Human kidney lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 7 : Human pancreas lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 8 : Human heart lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) ( ab6721 ) at 1/5000 dilution Performed under reducing conditions. Exposure time : 5 minutes A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230).