All lanes : Anti-PTPN13 antibody ( ab10867 ) at 2 µg/ml Lane 1 : 0.1µg recombinant PTP-BAS (PTPN13) Lane 2 : 0.1µg recombinant PTPH1 (PTPN3) Lane 3 : 0.1µg recombinant STEP (PTPN5) Lane 4 : 0.1µg recombinant SHP2 (PTPN11) Lane 5 : 0.1µg recombinant BDP1 (PTPN18) Performed under reducing conditions. Predicted band size : 277 kDa Observed band size : 58 kDa ( why is the actual band size different from the predicted? ) Recombinant PTP phosphatase domains (0.1 µg/lane) were probed with ab10867 at 2ug/ml. The antibody recognises PTP-BAS (PTPN13) GST fusion protein at ~58kDa. Recombinant PTP phosphatase domains provided by PurelyProteins