TNF Receptor I protein (Fc Chimera Active)
| Name | TNF Receptor I protein (Fc Chimera Active) |
|---|---|
| Supplier | Abcam |
| Catalog | ab83577 |
| Category | Protein |
| Prices | $315.00 |
| Sizes | 20 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | Activity: The ND 50 of ab83577 is typically 15-30 ng/ml as measured by its ability to neutralize TNF-a mediated cytoxicity in murine WEHI 164 cells in the presence of actinomycin D. |
| SwissProt/Accession | P19438 |
| Gene | TNFRSF1A |
| Residue | 22 to 209 |
| Sequence | Theoretical Sequence: IYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCT KCHKGTYLY NDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKE MGQVEISSC TVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHL SCQEKQNTV CTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKG TEDSGIPKV DKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDT LMISRTPEV TCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNS TYRVVSVLT VLHQDWLNGKEYKCRVSNKALPAPIEKTISKAKGQPREP QVYTLPPSR DELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTT PPVLDSDGS FFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLS LSPGK |
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Product images
Densitometry scan demonstrating the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83577 before and after treatment with glycosidases to remove oligosaccharides. Lane 1 – MW markers; Lane 2 – ab83577 ; Lane 3 – ab83577 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83577 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein loaded per lane. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Slight drop in MW after treatment with glycosidase cocktail suggests presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.