Active human UBE2V1 full length protein
| Name | Active human UBE2V1 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198472 |
| Category | Protein |
| Prices | $359.00 |
| Sizes | 100 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | His tag N-Terminus |
| Bioactivity | 50 μl reaction mix (4 mM ATP, 5 mM MgCl 2 , 1 mM DTT, UBE1 (0.5 μg), UEV1A or other E2, Biotin-Ubiquitin (0.5 μg) and Smurf2 (2 μg) in 50 mM Tris, pH 7.4) for 30 minutes at 37°C. Add to Streptavidin-coated plates for 30 minutes at 37°C. Incubate with anti-Smurf2, then HRP-labeled secondary antibody and chemiluminescent detection |
| SwissProt/Accession | Q13404 |
| Gene | UBE2V1 |
| Residue | 2 to 170 |
| Sequence | PGEVQASYLKSQSKLSDEGRLEPRKFHCKGVKVPRNFRLLEELEEGQKGV GDGTVSWGL EDDEDMTLTRWTGMIIGPPRTIYENRIYSLKIECGPKYP EAPPFVRFVTKINMNGVNSSN GVVDPRAISVLAKWQNSYSIKVVLQEL RRLMMSKENMKLPQPPEGQCYSN |
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